Undergraduate Catalog 2013-2014
CHM 377 Experimental Biochemistry
8 laboratory hours; 4 credits
Through a study of a commonly occurring genetic defect, this course introduces students to biochemical concepts and techniques used in current research. Techniques used include protein purification, enzymology, Western blotting, RNA isolation, DNA isolation, PCR-amplification of mutated regions of genes, cloning of PCR products into vectors, culturing of mammalian brain cells, immunocytochemistry, and retrieving and processing of genetic information using various databases and software packages.
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