College of Staten Island
 The City University of New York
 
  

Advanced Imaging Facility
 Asylum MFP 3D BIO Atomic Force Microscope


Reserve the Asylum MFP 3D BIO Atomic Force Microscope
Read the User Manual

Features:

  • Low Noise Scan Head and XY Scanner
  • 16µm Z range.
  • Superluminescent diode with an 860nm wavelength yields a flat baseline in force curves and images, and avoids interfering with red fluorophores (Texas Red, Cy5).
  • Closed loop operation. 90µm XY scan range.
  • X&Y sensor noise <0.6nm average deviation (Adev) in a 0.1Hz-1kHz bandwidth and sensor non-linearity <0.5% (Adev/full travel) at full scan. Z sensor noise <0.3nm Adev in a 0.1 Hz-1 kHz BW and Z sensor non-linearity less than 0.2% (Adev/full travel) at full scan.
  • Optical lever noise <0.03nm Adev in a 0.1Hz to 1kHz BW.
  • Built-in nanolithography and nanomanipulation capabilities with MicroAngelo™.

Stage:

  • The MFP-3D-BIO mounts on an inverted Zeiss Axio Observer.A1 microscope. AFM flexibly combines with many optical techniques such as bright field, phase, epifluorescence,TIRF, FRET, confocal, etc.
  • Accepts all high numerical objectives including those for TIRF.
  • Dual positioners control alignment between the tip, the optics, and the sample.
  • High resolution optical view allows easy positioning of the sample for AFM imaging.
  • Laser safety interlock is available for TIRF, confocal and laser-trapping experiments.

Optics:

  • The Top View module uses a Plan APO 10x/NA 0.28 objective integrated in the scan head and enables viewing of opaque samples, and easy alignment of the beam on the cantilever.
  • The phase contrast illumination module utilizes the same objective for use as a condenser lens.

Environmental Accessories for Dynamic Experiments:

  • BioHeater heats samples to 80°C with an accuracy of 0.1°C
  • Closed fluid cell with 10 ports allows imaging in a completely sealed environment.
  • PolyHeater (non-liquid samples only) heats samples to 300°C with an accuracy of 0.3°C.

Flexible Software:

  • User-friendly interface built on IGOR Pro integrates analysis, scripting and publication-quality graphics.
  • Built-in, easy cantilever calibration by the thermal or Sader methods.
  • Measure force curves at specified locations on your sample with a simple click of the mouse.
  • Process images using auto-generated masks to eliminate artifacts.
  • Add fit equations to section plots, histograms or any other data set.
  • ARgyle™ software module (which comes standard), allows users to render images in advanced 3D mode, as stereo anaglyphs (3D glasses), or as overlays by combining separate channels of data into a single 3D image (ie. confocal data as color scale on a 3D AFM topography image).
  • System includes computer with competitive specifications and dual-monitor 3D graphics card.