College of Staten Island
 The City University of New York
 
  

Advanced Imaging Facility
 Leica SP2 AOBS Confocal Microscope


Reserve the Leica SP2 AOBS Confocal Microscope
Read the User Manual

Capabilities:

  • Imaging UV and VIS fluorophores in fixed or live samples
  • Spectral imaging
  • Timelapse imaging
  • 3D z-stacks
  • FRAP/FLIP, FRET

Microscopes:

  • Leica DM IRE2 (inverted)
  • Leica DM RXA2 (upright)

Lasers:

  • 405 nm Diode laser
  • Argon/2 (458, 476, 488, 496, 514nm)
  • HeNe 543, 594 nm
  • HeNe 633nm

Conventional fluorescence filters:

  • Analyzer
  • Dapi
  • Green
  • Red

Objectives (upright):

  • 10x/0.30
  • 20x/0.70 (Leica Plan Apochromat)
  • 40x/1.25-0.75 oil (Leica Plan Apochromat)
  • 40x/0.8 water-immersion (Leica Plan Apochromat)
  • 63x/1.2 (Leica Plan Apochromat)
  • 63x/1.4-0.60 oil (Leica Plan Apochromat)

Objectives (inverted):

  • 10x/0.30 (Leica Plan Fluotar)
  • 40x/1.25-0.75 oil (Leica Plan Apochromat)
  • 63x/1.4-0.60 oil (Leica Plan Apochromat)

Dichroic(s):

  • AOBS serves as tunable dichroic for all lasers

Detectors:

  • Three channels
  • All cooled PMTs
  • All spectral detactors
  • Transmitted light detector