Registration is open for the 19th Annual Workshop on FLIM and FRET Microscopy, March 9-13, 2020.

http://www.kcci.virginia.edu/workshop
 
The Histochemical society is supporting TWO fellowships (each $1000) to attend the workshop which you can use it towards the workshop tuition fees. Please visit the web site for the 2019 award by clicking this link- http://histochemicalsociety.org/Awards/Awards.aspx .   Please visit the 2017 & 2018 awardees using the link- http://histochemicalsociety.org/Membership/Awards/Awardee-History/FRET-Workshop-Travel-Awardees.aspx
Also, limited amount of financial assistance is available. Hands-on instruction and training on wide-field, confocal, Spectral, and lifetime (FLIM) FRET imaging Microscopy systems. Discussion on best FLIM and FRET imaging and data analysis solutions. Personal attention for a maximum of 25 participants. Internationally recognized faculty.
 
Individual problem solving.

  • For attendees to experience an advanced practical course on Förster (fluorescence) resonance energy transfer FRET) and fluorescence lifetime imaging (FLIM) microscopy to image the protein-protein interactions.
  • Brief introductions to the principles of fluorescence, microscopy,  fluorophores, FRET and FLIM will be made. Emphasis willbe on practical aspects, hands-on individual instruction at the instruments followed by data analysis and interpretation.
  • Problem-solving discussions will address questions of fluorophore choices, the most suitable systems to achieve specific research objectives, qualitative vs. quantitative analysis and muchmore. Introduction for an unique image processing and analysis software (PFRET) will be given for training.
  • Live-cell specimens provided; participant's own specimens are welcome.

The AIF is a shared core facility under the Division of Science and Technology at CSI that houses advanced imaging systems including confocal, live-cell, transmission electron, scanning electron, and atomic force microscopes as well as dedicated lab space for specimen processing and analysis suites to allow researchers to prepare, image, and analyze samples at a single site. This facility is open to research scientists from all CUNY campuses. Scientists belonging to other academic institutions and industrial scientists are also invited to utilize the facility.

Contact Information

Dr. Greg Phillips
Facility Director
Phone : 718.982.3723
Contact Via Email
Dr. Michael Bucaro
Facility Manager
Phone : 718.982.3888
Contact Via Email

Recent Publications

Samaddar S, Vazquez K, Ponkia D, Toruno P, Sahbani K, Begum S, Abouelela A, Mekhael W, Ahmed Z. Transspinal direct current stimulation modulates migration and proliferation of adult newly born spinal cells in mice. J Appl Physiol (1985). 2017 Feb 1;122(2):339-353. doi: 10.1152/japplphysiol.00834.2016. Epub 2016 Dec 8. PubMed PMID: 27932680. 

Alonso AD, Cohen LS, Corbo C, Morozova V, ElIdrissi A, Phillips G, Kleiman FE. Hyperphosphorylation of Tau Associates With Changes in Its Function Beyond Microtubule Stability. Front Cell Neurosci. 2018 Oct 9;12:338. doi: 10.3389/fncel.2018.00338. eCollection 2018. Review. PubMed PMID: 30356756; PubMed Central PMCID: PMC6189415. 

Mukherjee S, Fried A, Hussaini R, White R, Baidoo J, Yalamanchi S, Banerjee P. Phytosomal curcumin causes natural killer cell-dependent repolarization of glioblastoma (GBM) tumor-associated microglia/macrophages and elimination of GBM  and GBM stem cells. J Exp Clin Cancer Res. 2018 Jul 25;37(1):168. doi: 10.1186/s13046-018-0792-5. PubMed PMID: 30041669; PubMed Central PMCID: PMC6058381. 

LaMassa N, Arenas-Mena C, Phillips GR. Electron microscopic characterization of nuclear egress in the sea urchin gastrula. J Morphol. 2018 May;279(5):609-615. doi: 10.1002/jmor.20796. Epub 2018 Jan 31. PubMed PMID: 29383750. 

Suyama K, Yao J, Liang H, Benard O, Loudig OD, Amgalan D, McKimpson WM, Phillips GR, Segall J, Wang Y, Fineberg S, Norton L, Kitsis RN, Hazan RB. An Akt3 Splice Variant Lacking the Serine 472 Phosphorylation Site Promotes Apoptosis and Suppresses Mammary Tumorigenesis. Cancer Res. 2018 Jan 1;78(1):103-114. doi: 10.1158/0008-5472.CAN-15-1462. Epub 2017 Oct 16. PubMed PMID: 29038347. 

Mukherjee S, Hussaini R, White R, Atwi D, Fried A, Sampat S, Piao L, Pan Q, Banerjee P. TriCurin, a synergistic formulation of curcumin, resveratrol, and epicatechin gallate, repolarizes tumor-associated macrophages and triggers an immune response to cause suppression of HPV+ tumors. Cancer Immunol Immunother. 2018 May;67(5):761-774. doi: 10.1007/s00262-018-2130-3. Epub 2018 Feb 16. PubMed  PMID: 29453519. 

News and Events