All cells require a membrane to separate them from their neighboring cells or the environment. While this compartmentalization is essential for life and its processes, it also poses problems: Not only do chemicals need to cross the barrier to get in and out of the cell, but communication also needs to take place between the cell and its environment. These transport and communication tasks are being carried out by proteins in the membrane that form transporters, pores, channels and signal transducers.
Research in Dr. Poget’s lab focuses on understanding a few of these membrane proteins and their function on an atomic level of structural detail. We employ nuclear magnetic resonance spectroscopy (NMR) and a range of ancillary biophysical techniques to study these proteins. While the main focus in the lab is on understanding the biological systems under study, significant effort is also devoted to developing the NMR methodology to tackle these questions.
Ph.D in Chemsitry, University of Cambridge, UK (2001)
Diploma in Chemistry, University of Basel, Switzerland (1997)
Scholarship / Publications
G. B. Legge, S. F. Poget, M. R. Proctor, S. M. V. Freund, M. Bycroft: 1H, 13C and 15N NMR assignments of the C-type lectin TC14. J. Biomol. NMR 2000, 18 283-284.
S. F. Poget, S. M. V. Freund, M. J. Howard, M. Bycroft: The ligand-binding loops in the tunicate C-type lectin TC14 are rigid. Biochemistry 2001, 40 10966-10972.
S. F. Poget, S. T. Krueger-Koplin, R. D. Krueger-Koplin, S. M. Cahill, S. Chandra Shekar, M. E. Girvin: Letter to the Editor: NMR assignment of the dimeric S. aureus small multidrug-resistance pump in LPPG micelles. J. Biomol. NMR 2006, 36 (Supplement 5) 10.
S. F. Poget, S. M. Cahill, M. E. Girvin: Isotropic bicelles stabilize the functional form of a small multidrug-resistance pump for NMR structural studies. J. Am. Chem. Soc. 2007, 129 2432-2433.
S. F. Poget, M. E. Girvin: Solution NMR of membrane proteins in bilayer mimics: Small is beautiful, but sometimes bigger is better. Biochim. Biophys. Acta 2007, 1768, 3098-106.